Publications

Cover arts

2025-2010

  1. G Bojanov, G., C Garcia-Sanz, C., Palomo*, JM. Colloidal Protein–Silver Nanoparticle Metalloenzyme as Artificial Redox Biocatalyst.Catalysts 2025 15 (1), 61. doi.org/10.3390/catal15010061

  2. Ortega-Nieto, C., Losada-Garcia, N., Domingo-Calap,P., Pawlyta, M., Palomo* J.M. Low Silver/Copper Exchange in a Copper-Phosphate Enzyme Nanoflower Hybrid Extremely Enhanced Antimicrobial Efficacy against Multidrug Resistant Bacteria. ACS Applied Bio Materials 2024, 7 (10), 6740-6748. doi.org/10.1021/acsabm.4c00898

  3. Garcia-Sanz, C., Palomo*, J.M. Zinc Nanobiohybrids for the Photocatalytic Conversion of CO2 to Bicarbonate. ACS Sustain..Chem. Eng. 2024, 12 (36), 13678-13686.doi.org/10.1021/acssuschemeng.4c05893
  4. Ortega-Nieto, C., Salta, M.,  Noël-Hermes, N.,  Palomo*, JM.Metal Bionanohybrids against Microbiologically Influenced Corrosion (MIC) Consortia. Nanomaterials 2024, 14 (17), 1376.doi.org/10.3390/nano14171376
  5.  Nuti, S.,  Fernández-Lodeiro, J., Palomo, J.M., Capelo-Martinez, J-L., Lodeiro, C.,  Fernández-Lodeiro, A.Synthesis, Structural Analysis, and Peroxidase-Mimicking Activity of AuPt Branched Nanoparticles. Nanomaterials 2024, 14 (13), 1166. doi.org/10.3390/nano14131166
  6.  Garcia-Sanz, C., Andreu, A., Pawlyta, M., Vukoičić, A., Milivojević, A., de Las Rivas, B.,  Bezbradica, D., Palomo*, JM. Artificial manganese metalloenzymes with laccase-like activity: design, synthesis, and characterization. ACS Applied Bio Materials 2024, 7 (7), 4760-4771..doi.org/10.1021/acsabm.4c00571
  7. Garcia-Sanz, C. Guijarro,L. Pawlyta, M .,Palomo*, JM. Mechanochemical synthesis of Zn-bionanohybrids: size effect at the nanoscale to improve their enzyme-like activity. RSC Mechanochem., 2024, 1, 219-227. doi.org/10.1039/D4MR00019F  (Cover art)
  8. Bezbradica, D.; van den Bogaard, E.; Palomo, J.M. Abboud, K. Y.Twinning for intensified enzymatic processes for production of prebioticcontaining functional food and bioactive cosmetics (TwinPrebioEnz).Chemical Industry / Hemijska Industrija, 2024, 78, p74. 
  9.  Sofia Santos, A., Losada‐Garcia, N., Garcia‐Sanz, C.,  Silva, AMS., Marques, MMB, Palomo,* JM.Exploring Thermomyces lanuginosus Lipase (TLL)‐PdNPs Nanohybrid as Suitable Catalyst for One‐pot Synthesis of Bis(3‐indolyl)phenylmethane.ChemCatChem 2024, 16 (3), e202300748. doi.org/10.1002/cctc.202300748
  10. Aguilera-Rodriguez,  D., Ortega-Alarcon, D.,  Vazquez-Calvo, A., Ricci, V., Abian, O.,  Velazquez-Campoy, A.,  Alcami, A.,Palomo,* JM.Inhibition of SARS-CoV-2 3CLpro by chemically modified tyrosinase from Agaricus bisporus. RSC Med Chem. 2024, 15 (12), 4159-4167. doi.org/10.1039/D4MD00289J
  11. C Garcia-Sanz, B de Las Rivas, JM Palomo. Design of a gold nanoparticles site in an engineered lipase: an artificial metalloenzyme with enantioselective reductase-like activity. Nanoscale 2024, 16 (14), 6999-7010. doi.org/10.1039/ D4NR00573B.
  12. Andreu, A.,  Ćorović, M., Garcia-Sanz, C., Santos, A.S., Milivojević, A., Ortega-Nieto,C., Mateo, C., Bezbradica, D., Palomo,* JM. Enzymatic glycosylation strategies in the production of bioactive compounds.Catalysts 2023, 13 (10), 1359.doi.org/10.3390/catal13101359
  13. Rodríguez-Otero, A.,Losada-García, N., Guerra-Rodríguez, S., Palomo*, JM, Rodríguez-Chueca, J.Antibacterial effect of metal-enzyme hybrid nanomaterials. J. Environ.Chem. Eng.2023, 11 (5), 110499.doi.org/10.1016/j.jece.2023.110499
  14. Palomo*, JM. Preparation of Dual‐activity Enzyme‐metal‐nanoparticles Conjugate Catalysts for Cascade Processes. ChemCatChem 2023, 15 (18), e202300701. doi.org/10.1002/cctc.202300701
  15. Ortega-Nieto, C., Losada-Garcia, N., Prodan, D.,Furtos, G.,Palomo*, JM. Recent Advances on the Design and Applications of Antimicrobial Nanomaterials. Nanomaterials 2023, 13 (17), 2406. doi.org/10.3390/nano13172406
  16. Losada-Garcia, N., Vazquez-Calvo, A.,Ortega-Alarcon, D.,Abian, O., Velazquez-Campoy, A., Domingo-Calap, P., Alcami, A.,Palomo*, JM.Nanostructured biohybrid material with wide-ranging antiviral action. Nano Research, 2023, 13 (17), 2406. doi.org/10.1007/s12274-023-5765-0 
  17. Losada‐Garcia, N. Urriolabeitia, EP.,  Palomo*, JM. Solid‐Phase Lipase‐CuNPs Biohybrids as Catalysts for One‐Pot Parallel Synthesis of 2, 3, 4‐Triacetyl‐D‐Gluconic Acid. ChemCatChem 2003, 15 (9), e202201632.doi.org/10.1002/cctc.202201632 
  18. Losada-Garcia, N., Vazquez-Calvo, Alcami, A., Palomo*, JM. Preparation of Highly Stable and Cost-Efficient Antiviral Materials for Reducing Infections and Avoiding the Transmission of Viruses such as SARS-CoV-2. ACS Appl. Mat. Interfaces, 2023, 15 (18), 22580-22589. doi.org/10.1021/acsami.3c03357
  19. Ortega-Nieto, C., Losada-Garcia, C., Pessela, B.C., Domingo-Calap, P., Palomo*, JM. Design and synthesis of copper nanobiomaterials with antimicrobial properties. ACS bio & med Chem Au. 2023,  3, 4, 349–358. doi.org/10.1021/acsbiomedchemau.2c00089
  20. Losada-Garcia, N., Carranza, J., Palomo*, JM. Graphene-TLL-Cu2ONPs Hybrid as Highly Efficient Catalyst for Degradation of Organic Compounds. Nanomaterials 2023, 13 (3), 449. doi.org/10.3390/nano13030449
  21. Fernández-Lodeiro, A., Fernández Lodeiro, J., Losada-Garcia, N., Nuti, S., Capelo-Martinez, J.L:, Palomo*, J.M., Lodeiro, C.Copper (I) as a reducing agent for the synthesis of bimetallic PtCu catalytic nanoparticles. Nanoscale Adv. 2023,  5 (2), 513-521.doi.org/10.1039/D3NA00158J
  22. Losada-Garcia, N., Santos, A.S.,  Marques, M.M.B.,  Palomo*, J.M. Temperature-induced formation of Pd nanoparticles in heterogeneous nanobiohybrids: application in C–H activation catalysis. Nanoscale Adv. 2023, 5 (2), 513-521.doi.org/10.1039/D2NA00742H. 
  23. Losada‐Garcia, N. Urriolabeitia, EP.,  Palomo*, JM.Metal nanoparticles incorporated within graphene-enzyme preparations for synergistic multiactive catalysts.ACS Appl. Nano Mat. 2023, 6 (1), 704-713. doi.org/10.1021/acsanm.2c04963
  24. Palomo*, J.M. Synthesis of Regioselective Carbohydrates Building Blocks by Heterogeneous Engineered Biocatalysts.General Chem. 2022, 8 (3-4), 220009.
  25. Naapuri, J.M., Losada‐Garcia, N., Alexander Rothemann, R., Manuel Carmona Pichardo, M., Prechtl, M.H.G., Palomo*, J.M., Deska, J.Cascade Catalysis Through Bifunctional Lipase Metal Biohybrids for the Synthesis of Enantioenriched O‐Heterocycles from Allenes. ChemCatChem 2022, 14 (16), e202200362.doi.org/10.1002/cctc.202200362
  26. Losada-Garcia, N.,  Rodriguez-Otero, A.,  Ortega-Nieto, C., Azarmi, Ariane, Palomo, JM.Catalase like-activity of metal NPs–enzyme biohybrids. Appl. Nano 2022, 3 (3), 149-159. doi.org/10.3390/applnano3030011
  27. Naapuri, J.M., Losada‐Garcia, N., Palomo*, J.M., Deska, J.Synthesis of silver and gold nanoparticles–enzyme–polymer conjugate hybrids as dual-activity catalysts for chemoenzymatic cascade reactions. Nanoscale 2022, 14 (15), 5701-5715. doi.org/10.1039/D2NR00361A.
  28. Naapuri, J.M.,  Åberg, G.A., Palomo, J.M., Deska, J. Arylative Allenol Cyclization via Sequential One-pot Enzyme & Palladium Catalysis. ChemCatChem,2021.13, 763-769.doi.org/10.1002/cctc.202001619
  29. Filice, M., Losada-Garcia, N., Perez-Rizquez, C., Marciello ,M., Morales, M.P., Palomo*, J. M. Palladium-Nanoparticles BioHybrids in Applied Chemistry.Appl. Nano, 2021. 2,1-13.doi.org/10.3390/applnano2010001               
  30. Palomo*, J.M. Artificial enzymes with multiple active sites Curr. Opin. Green. Sustain.Chem.2021, 29,100452.doi.org/10.1016/j.cogsc.2021.100452
  31. Losada-Garcia, N., Jimenez-Alesanco, A., Velázquez-Campoy, A.,  Abian, O., Palomo*, J. M. Enzyme/Nanocopper Hybrid Nanozymes: Modulating Enzyme-like Activity by the Protein Structure for Biosensing and Tumor Catalytic Therapy. ACS Appl. Mater Interfaces. 2021, 13,4, 5111-5124. doi.org/10.1021/acsami.0c20501
  32. Garcia-Sanz, C.,  Andreu, A., de las Rivas, B,  Jiménez, A.I.,  Pop, A., Silvestru, C.,  Urriolabeitia*, E.P., Palomo*,J.M. Pd-oxazolone complexes conjugated to an engineered enzyme: Improving fluorescence and catalytic properties.Org. Biomol.Chem. 2021. 19, 2773-2783.doi.org/10.1039/D1OB00305D
  33. Rodriguez-Oliva, I., Losada-Garcia, N., Santos, S., Marques*, M.M.B., Palomo*, J.M.Palladium nanocatalysts for Cascade C−N Cross‐Coupling/Heck Reaction.Asian J. Org. Chem. 2021. 10, 872-878.doi.org/10.1002/ajoc.202100077
  34. Brabcova J., Andreu, A., Aguilera, D., de las Rivas, B., Muñoz, R., Cabrera, Z., Palomo*, J. M. Geranyl Functionalized Materials for Site-Specific Co-Immobilization of Proteins.Molecules. 2021, 26,3028.doi.org/10.3390/molecules26103028
  35. Losada-Garcia, N.,  Andreu, A., Garcia-Sanz, C., Velasco-Torrijos, T., Palomo*, J. M. Glyconanomaterials for virus detection and inhibition.Nanomaterials, 2021, 11(7), 1684./doi.org/10.3390/nano11071684
  36. Palomo,* J.M., Galan, M.C., Garcia-Fernandez, J.M. Functional Glyconanomaterials.
    Nanomaterials, 2021,11(10), 2482. doi.org/10.3390/nano11102482
  37. Lopez-Tejedor, D., Clavería-Gimeno, R., Velazquez-Campoy, A., Abian, O.,  Palomo,* J.M. Tyrosinase from mushroom Agaricus bisporus as an inhibitor of the Hepatitis C virus. Pharmaceuticals  2021,14 (8), 759 https://doi.org/10.3390/ph14080759
  38. Palomo,* J.M.Editorial overview: Synthetic enzymes: a key tool in sustainable chemistry. Curr. Opin. Green. Sustain. Chem. 2021,32,100552. https://doi.org/10.1016/j.cogsc.2021.100552. 

  39. Tacin, M.V., Costa-Silva, A., de Paula, A.V., Palomo*, JM, Santos-Ebinuma, V.C.. Microbial lipase: A new approach for a heterogeneous biocatalyst. Preparative Biochemistry & Biotechnology 2021, 51 (8), 749-760.doi.org/10.1080/ 10826068.2020.1855442

  40. Palomo,* J.M., Urriolabeitia, E.P.. Protein-Pd Complexes Conjugates as Artificial Enzymes. ‎Gen. Chem. 2020, 6, 190030.
  41. Losada-Garcia, N., Rodriguez-Otero, A., Palomo,* J.M. Tailorable synthesis of heterogeneous enzyme–copper nanobiohybrids and their application in the selective oxidation of benzene to phenol. Catal.Sci.Technol., 2020, 10, 196-206. doi.org/10.1039/C9CY02091H

  42.  Losada-Garcia, N., Rodriguez-Oliva, I., Simovic, M., Bezbradica, D.I.,  Palomo,* J.M. New Advances in Fabrication of Graphene Glyconanomaterials for Application in Therapy and Diagnosis.ACS Omega, 2020, 5,4362-4369. doi/10.1021/acsomega.9b04332

  43. Palomo,* J.M.  Synthetic complexity created by lipases. Nature Catal., 2020, 3, 335-336. doi.org/10.1038/s41929-020-0453-x 
  44. Losada-García, N., Rodriguez-Otero, A., Palomo*, J.M., Fast Degradation of Bisphenol A in water by nanostructured CuNPs@CAL-B biohybrid catalyst. Nanomaterials. 2020, 10, 7.   doi.org/10.3390/nano10010007
  45. Benavente,R., Lopez-Tejedor,D., Morales, M.P,   Perez-Rizquez, C.,  Palomo* J.M. The enzyme-induced formation of iron hybrid nanostructures with different morphologies.
    Nanoscale, 2020,12, 12917-12927. doi.org/10.1039/D0NR03142A
  46. McCarthy, C., Losada-García, N., Palomo*, J.M., Direct synthesis of phenols from phenylboronic acids in aqueous media catalyzed by a Cu(0)-nanoparticles biohybrid. Chemistry select 2020, 5, 7492-7496.  doi.org/10.1002/slct.202002110
  47. Losada-García, N., Rodriguez-Otero, A., Palomo*, J.M., High degradation of trichloroethylene in water by nanostructured MeNPs@CALB biohaybrid catalysts. Catalysts. 2020, 10(7), 753. doi.org/10.3390/catal10070753
  48. Jaramillo, J.,  Rodriguez-Oliva, I., Abian, O., Palomo*, J.M.. Specific chemical incorporation of L-DOPA and functionalized
    L-DOPA-hyaluronic acid in Candida antarctica lipase: creating potential mussel-inspired. SN Applied Sciences, 2020, Article number: 173. doi.org/10.1007/s42452-020-03545-w
  49. Losada-Garcia, N., Cabrera, Z., Urrutia P., Garcia-Sanz, C., Andreu, A., Palomo*,J.M. Recent Advances in Enzymatic and Chemoenzymatic Cascade Processes. Catalysts. 2020, 10, 1258  doi.org/10.3390/catal10111258. 
  50. Fernández-Lodeiro, A.; Djafari, J.; Lopez-Tejedor, D.; Perez-Rizquez, C.; Rodríguez-González, B.; Capelo, J.L.; Palomo*, J.M.; Lodeiro, C., Fernández-Lodeiro, J. Highly accessible aqueous synthesis of well-dispersed dendrimer type platinum nanoparticles and their catalytic applications. ‎Nano Res. 2019, 12, 1083. doi.org/10.1007/s12274-019-2350-7.
  51. Perez-Rizquez, C.; Lopez-Tejedor, D.; Plaza-Vinuesa, L.; de las Rivas, B.; Muñoz, R.; Cumella, J.; Palomo*, J.M. Chemical Modification of Novel Glycosidases from Lactobacillus plantarum Using Hyaluronic Acid: Effects on High Specificity against 6-Phosphate Glucopyranoside.Coatings 2019, 9, 311  doi.org/10.3390/coatings9050311.
  52. Perez-Rizquez, C.;Rodriguez-Otero, A.; Palomo,* J.M., Combining enzymes and organometallic complexes: novel artificial metalloenzymes and hybrid systems for C-H activation chemistry. Org.Biomol.Chem. 2019,17, 7114 7123 doi.org/10.1039/C9OB01091B
  53. Palomo,* J.M., Nanobiohybrids: a new concept for metal nanoparticles synthesis. Chem. Commun. 2019. 55, 9583-9589. doi.org/10.1039/C9CC04944D.
  54. Losada-Garcia, Noelia; Berenguer-Murcia, Angel; Cazorla-Amorós, Diego; Palomo,* J.M., Efficient production of multi-layer graphene from graphite flakes in water by lipase-graphene sheets conjugation. Nanomaterials. 2019.9(9), 1344 doi.org/10.3390/nano9091344
  55. Perez-Rizquez, C., Abian, O., Palomo,* J.M., Site-Selective modification of Tryptophan and Protein Tryptophan residues through PdNPs bionanohybrid-catalysed C-H Activation in Aqueous media. ‎Chem. Commun. 2019, 55, 12928-12931. doi.org/10.1039/C9CC06971B
  56. Lopez-Tejedor, D., Palomo,* J.M. Efficient purification of a highly active H-subunit of tyrosinase from Agaricus bisporus.
    ‎Protein Expr. Purif . 2018, 145, 64-70.  doi.org/10.1016/j.pep.2018.01.001.

  57. Romero, O, de las Rivas, B., Lopez-Tejedor,D., Palomo,* J.M. Effect of Site-Specific Peptide-Tag Labeling on the Biocatalytic Properties of Thermoalkalophilic Lipase from Geobacillus thermocatenulatus.ChemBioChem. 2018. 19, 369-378.  doi.org/10.1002/cbic.201700466

  58. Lopez-Tejedor, D., Benavente, R., Palomo,* J.M. Iron Nanostructured catalysts: Design and applications.
    ‎Catal Scien.Technol 2018, 8, 1754-1776. doi.org/10.1039/C7CY02259J
  59. Benavente, R., Lopez-Tejedor, D., Palomo,* J.M. Synthesis of a superparamagnetic ultrathin FeCO3 nanorods–enzyme bionanohybrid as a novel heterogeneous catalyst. ‎ChemCommun 2018, 54, 6256-6259. doi.org/10.1039/C8CC02851F
  60. Benavente, R., Lopez-Tejedor, D., Perez-Rizquez, C., Palomo,* J.M. Ultra-Fast Degradation of p-Aminophenol by a Nanostructured Iron Catalyst. Molecules 2018, 23, 2166. doi.org/10.3390/molecules23092166
  61. O´Brien, P., Lopez-Tejedor, D., Benavente, R., Palomo,* J.M. Pd nanoparticles-Polyethylenemine-Lipase Bionanohybrids as heterogeneous catalysts for selective oxidation of aromatic alcohols. ‎ChemCatChem 2018, 10, 5006–5013.  doi.org/10.1002/cctc.201801294
  62. Lopez-Tejedor, D., de las Rivas, B., Palomo,* J.M. Ultra-small Pd(0) nanoparticles into a designed semisynthetic lipase: an efficient and recyclable heterogeneous biohybrid catalyst for the Heck reaction under mild conditions.
    ‎Molecules 2018, 23(9), 2358. doi.org/10.3390/molecules23092358
  63. Mateo, C., Palomo,* J.M. Asymmetric and Selective Biocatalysis. Catalysts 2018,8 (12), 588.doi.org/10.3390/catal8120588
  64. Filice, M., Aragon C., Mateo C., Palomo,* J.M., Enzymatic transformations in food chemistry.Curr. Org. Chem. 2017, 21 (2), 139-148
  65. Callaghan, C., Redmond, M., Alnoch, R.C., Mateo, C., Filice, M.,Palomo,* J.M. Biocatalytic process optimization for production of high-added value 6-O-hydroxy and 3-O-hydroxy glycosyl building blocks.ChemCatChem. 2017.9, 2536-2543. doi.org/10.1002/cctc.201601632
  66. Palomo,* J.M. Semisynthetic Enzymes by Protein–Peptide Site-Directed Covalent Conjugation: Methods and Applications.
    Methods in Enzymology. 2017. 590, 305-316.
  67. Filice, M., Molina M., Benaiges M.D., Abian, O., Valero F, Palomo,* J.M. Solid-surface activated recombinant Rhizopous oryzae lipase expressed in Pichia Pastoris and chemically modified variants as efficient catalysts in the synthesis of hydroxy monodeprotected glycals. Catal. Sci. Technol. 2017, 7, 1766-1775.doi.org/10.1039/C7CY00255F. 
  68. Neville, A., Iniesta J., Palomo,* J.M., Design of heterogeneous Hoveyda-Grubbs Second-generation catalyst-lipase conjugates.
    Molecules.2016, 21, 1680. doi.org/10.3390/molecules21121680
  69. Alnoch, R., Rodrigues de Melo, R., Palomo, J.M.,Maltempi de Souza, E., Krieger,N., Mateo C.New Tailor-Made Alkyl-Aldehyde Bifunctional Supports for Lipase Immobilization.Catalysts. 2016,6, 191.  doi.org/10.3390/catal6120191
  70. Dunne, A.,Palomo,* J.M., Efficient and green approach for the complete deprotection of O-acetylated biomolecules.
    RSC advances. 2016,6, 88974-88978.  doi.org/10.1039/C6RA19645D
  71. Rivero C.W.,Palomo,* J.M., Covalent immobilization of Candida rugosa lipase at alkaline pH and their application in the regioselective deprotection of per-O-acetylated thymidine transformations. Catalysts. 2016,6(8), 115. doi.org/10.3390/catal6080115
  72. Brabcova, J., Blazek, J. Krecmerova, M., Vondrášek J, Palomo*, J. M., Zarevucka, M.Regioselective palmitoylation of 9-(2,3-dihydroxypropyl)adenine catalysed by a glycopolymer-enzyme conjugate. Molecules.2016,21(5), 648. doi.org/10.3390/molecules21050648
  73.  Palomo*, J.M., Filice, M. Biosynthesis of Metal Nanoparticles: Novel Efficient Heterogeneous Nanocatalysts
    Nanomaterials 2016, 6(5), 84. doi.org/10.3390/nano6050084
  74. Cuenca, T., Filice, M., Palomo*, J.M.Palladium nanoparticles enzyme aggregate (PANEA) as efficient catalyst for Suzuki-Miyaura reaction in aqueous media. Enzyme Microb. Technol. 2016, 95,242-247. doi.org/10.1016/j.enzmictec.2016.01.014
  75. Aragon C., Palomo, J.M., Filice, M.,Mateo C. Immobilization of Aldolase for C-C Bond Formation
    Curr. Org. Chem. 2016, 20,1243-1251.
  76. Palomo*, J.M, .Filice, M. New emerging bio-catalysts design in biotransformations.
    Biotechnol Adv.2015,33, 605-613.doi.org/10.1016/j.biotechadv.2014.12.010
  77. Branco, R. V., Gutarra, M. L. E., Guisan, J. M., Freire, D. M. G., Almeida R. V., Palomo, J.M. Improving the Thermostability and Optimal Temperature of a Lipase from the Hyperthermophilic Archaeon Pyrococcus furiosus by Covalent Immobilization.
    BioMed Research International, vol. 2015, Article ID 250532, 8 pages, 2015.  doi.org/10.1155/2015/250532
  78. Filice, M.,Romero O, Gutierrez-Fernandez, J., de las Rivas B., Hermoso J.A., Palomo*, J.M. Synthesis of a heterogeneous artificial metallolipase with chimeric catalytic activity. Chem. Commun .2015, 51, 9324-9327.doi.org/10.1039/C5CC02450A
  79. Quintana, P.G., Guillen, M., Marciello, M., Valero, F; Palomo, J.M., Baldessari, A. Enzyme-catalyzed preparation of chenodeoxycholic esters by an immobilized heterologous Rhizopus oryzae lipase.
    J. Mol. Catal B: Enzym.2015,118,36-42. doi.org/10.1016/j.molcatb.2015.05.008
  80. Filice, M.,Romero O, Aires, A., Guisan, J.M., Rumbero, A.,Palomo*, J.M.Preparation of an immobilized lipase-palladium artificial metalloenzyme as catalyst in Heck reaction: role of the solid-phase.
    Adv. Synth. Catal.2015,357,2687-2696. doi.org/10.1016/j.molcatb.2015.05.008
  81. Filice, M, Palomo*, J. M. Cascade reactions catalyzed by bionanostructures.. ACS Catalysis. 2014, 4, 1588-1598.doi.org/10.1021/cs401005y
  82. Brabcova, J.,Carrasco-Lopez, C., Bavaro T., Hermoso, J.A., Palomo*, J. M. Escherichia coli LacZ β-galactosidase inhibition by monohydroxy acetylated glycopyranosides: Role of the acetyl groups.
    J Mol Catal B: Enzym. 2014, 107, 31-38.doi.org/10.1016/j.molcatb.2014.05.008
  83. Palomo*, J. M. Solid-phase peptide synthesis: an overview focused on the preparation of biologically relevant peptides.
    RSC Advances. 2014, 4, 32658-32672.doi.org/10.1039/C4RA02458C
  84. Filice, M., Romero O, Abian, O., de las Rivas B., Palomo*, J.M. Low ionic liquids concentration in water: a green and simple approach to improve activity and selectivity of lipases. RSC Advances.2014, 4, 49115-49122.  doi.org/10.1039/C4RA07625G
  85. Godoy, C., de las Rivas, B., Mateo, C., Fernandez-Lorente, G; Guisan, J. M., Palomo*, J.M. Changes on enantioselectivity of a genetically modified thermophilic lipase by site-directed oriented immobilization. J Mol Cata B: Enzym. 2013, 87,121-127.  doi.org/10.1016/j.molcatb.2012.10.003
  86. de Lima, L.N., Aragon, C.C., Mateo, C., Palomo, J.M, Giordano, R.L.C., Tardioli, P.W., Guisan, J.M., Fernandez-Lorente, G. Immobilization and stabilization of a bimolecular aggregate of the lipase from Pseudomonas fluorescens by multipoint covalent attachment. Process Biochem. 2013, 48, 118-123.doi.org/10.1016/j.procbio.2012.11.008 
  87. Romero O, Rivero C.W., Guisan, J.M., Palomo*, J. M. Novel enzyme-polymer conjugates for biotechnological applications
    PeerJ,2013,:e27 doi.org/10.7717/peerj.27. 
  88. Palomo*, J. M. Click-chemistry in Biocatalysis
    Curr.Org Chem. 2013, 17, 691-700.
  89. Brabcova, J., Filice, M. , Gutarra, M., Palomo*, J. M. Synthesis of enantiopure drugs and drug intermediates by immobilized lipase-catalysis Curr. Bio.Compd. 2013, 9, 113-136.10.2174/22115528112019990009
  90. Filice, M., Marciello ,M., Morales, M.P., Palomo*, J. M. Synthesis of heterogeneous enzyme-metal nanoparticle biohybrids in aqueous media and their applications in C-C bond formation and tandem catalysis.Chem. Commun. 2013, 49 (61), 6876-6878. doi.org/10.1039/C3CC42475H
  91. Palomo*, J. M., Filice, M. ,Romero O., Guisan J.M. Improving lipase activity by immobilization and post-immobilization strategies
    Methods in Molecular Biology 2013, 1051, 255-273.doi.org/10.1007/978-1-62703-550-7_17
  92. Brabcova, J.,Demianova, Z., Vondrasek, J. Jagr, M., Zarevucka, M., Palomo*, J. M. Highly selective purification of three lipases from Geotrichum candidum 4013 and their characterization and biotechnological applications. J Mol Cata B: Enzym.2013, 98, 62-72.doi.org/10.1016/j.molcatb.2012.10.003
  93. Fernandez-Lorente, G; Betancor, L; Carrascosa, A.V.; Palomo*, J. M., Guisan, J. M. Modulation of the selectivity of immobilized lipases by chemical and physical modifications: release of omega-3 fatty acids from fish oil. J. Am oil Food Chem. 2012, 89, 97-102. doi.org/10.1007/s11746-011-1885-x

  94. Filice M, Palomo*, J.M. Monosaccharide derivatives as central scaffolds in the synthesis of glycosylated drugs. RSC Advances. 2012, 2, 1729-1742. doi.org/10.1039/C2RA00515H. Highlight in ChemInform 2012 43 (1)

  95. Palomo*, J. M., Guisan, J.M. Different strategies for hyperactivation of lipase biocatalysts. Methods in Molecular Biology2012, 861, 329-341. 10.1007/978-1-61779-600-5_20
  96. Marciello, M., Filice M, Palomo*, J. M. Different strategies to enhance activities of lipase catalysts.
    Catal Sci.Technol. 2012, 2, 1531-1543. doi.org/10.1039/C2CY20125A. Highlight in ChemInform 2012 43 (42)
  97. Quintana, P.G., Guillen, M., Marciello, M., Valero, F, Palomo, J. M., Baldessari, A. Immobilized heterologous Rhizopus oryzae lipase as an efficient catalyst in the acetylation of cortexolone. Eur.J.Org.Chem., 2012, 23, 4306-4312. doi.org/10.1002/ejoc.201200178
  98. Romero O, Filice, M., de las Rivas B., Carrasco-Lopez, C., Klett, J., Morreale, A., Hermoso, J.A., Guisan, J.M., Abian, O., Palomo*, J.M.Semisynthetic Peptide-Lipase Conjugates for Improved Biotransformations. Chem.Commun.2012, 72, 9053-9055. doi.org/10.1039/C2CC34816K. Highlight inChemInform2012 43 (50).
  99. Filice M,Guisan, Jose M., Terreni, M., Palomo*, J. M. Regioselective monodeprotection of peracetylated carbohydrates. Nature Protoc.2012, 7, 1783-1796.doi.org/10.1038/nprot.2012.098
  100. Click reactions in protein chemistry: From the preparation of semisynthetic enzymes to new click enzymes
    Org Biomol.Chem. 2012, 10 , 9309-9318. doi.org/10.1039/C2OB26409A (Inside front cover)
  101. Cabrera Z, Palomo*, J. M. Enzymatic Desymmetrization of Prochiral Molecules. Curr. Org. Synth., 2012, 9, 791-805.

  102. Filice, M.,Romero, O, Guisan, J.M., Palomo*, J. M. Trans,trans-2,4-hexadiene incorporation on enzymes for site-specific immobilization and fluorescent labeling.Org Biomol.Chem. 2011,9, 5535-5540.doi.org/10.1039/C1OB05401E

  103. Gutarra M.L, Romero O., Abian, O., Torres, F.A.G., Freire, D.M.G., Castro, A.M., Guisan, J. M.,Palomo*, J.M. Enzyme surface glycosylation on the solid phase: improve activity and selectivity of Candida antarctica lipase B.
    ChemCatChem. 2011, 3, 1902-1910. doi.org/10.1002/cctc.201100211

  104. Cabrera Z, Palomo*, J.M. Enantioselective Desymmetrization of prochiral diesters catalyzed by immobilized Rhizopus oryzae lipase.Tetrahedron: Asymmetry. 2011, 22, 2080-2084.doi.org/10.1016/j.tetasy.2011.11.012

  105. Volpato, G., Filice, M., Ayub, M.A.Z., Guisan, J. M., Palomo*, J.M. Single-step Purification of different Lipases from Staphylococcus warneri. J. Cromatogr. A. 2010, 1217, 473-478.doi.org/10.1016/j.chroma.2009.11.055.
  106. Godoy, C.; de las Rivas, B.; Filice, M., Fernández-Lorente,G.,Guisan, J. M., Palomo*, J.M. Enhanced activity of an immobilized lipase promoted by site-directed chemical modification with polymers. Process Biochem. 2010,45, 534-541.doi.org/10.1016/j.procbio.2009.11.014. 
  107. Filice, M., Guisan, J. M., Palomo*, J.M. Recent Trends in regioselective Protection and deprotection of monosaccharides
    Curr. Org Chem. 2010,14, 516-532. Highlights in ChemInform 2010 41 (39)
  108. Cabrera,Z.; Gutarra M.L, Guisan, J.M.,Palomo*, J.M. Highly enantioselective biocatalysts by coating immobilized lipases with polyethyleneimine. Catal Commun. 2010,11,964-967.doi.org/10.1016/j.catcom.2010.04.010
  109. Filice, M.,Guisan, Jose M., Palomo*, J.M. Effect of Ionic Liquids as additives in the catalytic properties of different immobilized preparations of Rhizomucor miehei Lipase in the Hydrolysis of Peracetylated Lactal.
    Green Chem. 2010,12, 1365-1369. doi.org/10.1039/C003829F
  110. Palomo*, J.M. Diels-Alder cycloaddition in protein chemistry.
    Eur. J. Org Chem.2010,33,6303-6314.      doi.org/10.1002/ejoc.201000859. Highlights in ChemInform 2011 42 (11)

 

 

 

 

 

2009-2002

Filice, M; Bavaro T.; Fernandez-Lafuente,R.; Pregnolato M.; Guisan, J. M; *Palomo, J.M, Terreni,  M.
Chemo-Biocatalytic Regioselective Synthesis of Different Deprotected Monosaccharides.
Catal. Today . 2009, 140,11-18.

Cabrera,Z.; Fernández-Lorente,G., Fernandez-Lafuente,R.; Guisan, J. M.;Palomo*, J.M.
Enhancement of Novozym-435 catalytic properties by physical or chemical modification.
Process Biochem. 2009,44,226-231.

 Palomo*, J.M.
Modulation of enzymes selectivity via immobilization.
Curr. Org. Synth. 2009, 6,1-14

Carrasco-López, C.; Godoy, C.; de las Rivas, B.; Fernandez-Lorente, G.; Palomo, J.M., Guisán, J. M., Fernández-Lafuente, R.; Martínez-Ripoll M., Hermoso, J.A.
Activation of bacterial thermoalkalophilic lipases is spurred by dramatic structural rearrangements.
J. Biol.Chem.2009, 284,4365-4372.

Cabrera,Z.; Fernández-Lorente,G., Fernandez-Lafuente,R.; Palomo*, J.M; Guisan, J.M.
Novozym 435 displays very different selectivity compared to Candida antarctica B lipase adsorbed on standard hydrophobic supports.
J.Mol Cat B: Enzym 2009,57,171-176.

Rodrigues, D.S., Mendes, A.A., Filice, M., Guisan, J. M., Fernandez-Lafuente, R., Palomo*, J.M.
Different derivatives of a lipase display different regioselectivity in the monohydrolysis of per-O-acetylated 1-O-substituted-β-galactopyranosides
J.Mol Cat B: Enzym.2009,58,36-40.

 Filice, M., Vanna, R., Terreni, M., Guisan, J. M., Palomo*, J.M.
Lipase-Catalyzed Regioselective One-step Synthesis of Penta-O-acetyl-3-hydroxy-Lactal.
Eur. J. Org Chem.2009,20,3327-3329.

.Fernandez-Lorentes, G.; Filice, M; Terreni,  M.; Guisan, J. M; Fernandez-Lafuente,R., *Palomo, J. M.
Lecitase® ultra as regioselective biocatalyst in the hydrolysis of fully protected carbohydrates.Strong modulation by using different immobilization protocols.
J. Mol.Cat B: Enzym. 2008, 51,110-117

. Filice, M, Ubiali, D., Fernandez-Lafuente,R., Fernandez-Lorente, G., Guisan J. M, *Palomo, J. M, Terreni,  M.
A Chemo-Biocatalytic approach in the synthesis of β-O-Naphtylmethyl-N-Peracetylated Lactosamine.
J. Mol.Cat B: Enzym. 2008, 52-53, 106-112.

 Fernández-Lorente,G., Cabrera,Z.; Godoy, C.; Fernandez-Lafuente,R.; *Palomo, J. M, Guisan, J.M.
Interfacially activated Lipases against hydrophobic supports: effect of the support nature on the biocatalytic properties
Process Biochem. 2008, 43, 1061–1067.

 Cabrera,Z.; Fernández-Lorente,G., Palomo, J. M, Fernandez-Lafuente,R.; Guisan, J.M.
Asymmetric hydrolysis of di-methyl ester of phenylglutaric acid catalyzed by Lecitase Ultra®. Effect of the immobilization protocol on its catalytic properties.
Enzyme Microb Technol. 2008, 43, 531–536.

 Filice, M; Palomo, J. M, Bonomi, P.; Bavaro T.; Fernandez-Lafuente,R.; Guisan, J.M; Terreni,  M.
Preparation of linear oligosaccharides by a simple monoprotective chemoenzymatic approach
Tetrahedron. 2008,64, 9286-9292.

 Fernandez-Lorente, G., Godoy, C., Mendes, A. A., Lopez-Gallego,F, Grazu V,  de las  Rivas  B., Palomo J.M., Hermoso, J., Fernandez-Lafuente , R, Guisan J.M.
Solid-Phase Chemical Amination of Bacillus thermocatenulatus lipase to improve its stabilization via covalent immobilization on highly activated glyoxyl-agarose
Biomacromolecules. 2008,9, 2553–2561.

Palomo, J. M.
Lipases Enantioselectivity Alteration by Immobilization Techniques
Curr. Bio.Comp.. 2008,4, 126-138.

Mendes, A.A., Rodrigues, D. S., Filice, M., Fernandez-Lafuente, R., Guisan, J. M., *Palomo,J.M.
Regioselective monohydrolysis of per-O-acetylated 1-O-substituted-β-glucopyranosides catalyzed by immobilized lipases.
Tetrahedron. 2008,64,10721-10727.

Carrasco-López, C.; Godoy, C.; de las Rivas, B.; Fernandez-Lorente, G.; Palomo, J. M., Guisán, J. M., Fernández-Lafuente, R.; Martínez-Ripoll M., Hermoso, J.A.
Crystallization and preliminary X-ray diffraction studies of the BTL2 lipase from extremophilic microorganism Bacillus thermocatenulatus.
Acta Cryst. Sect F Struct Biol Cryst Commun.2008, 64, 1043–1045.

Palomo, J.M.; Segura, Rosa L.; Fernández-Lorente, G.; Guisán, J.M.; Fernández-Lafuente,R.
Glutaraldehyde modification of lipases adsorbed on aminated supports: A simple way to improve their behaviour as enantioselective biocatalyst.
Enzyme Microb Technol. 2007, 40,704-707.

Cabrera, Z.; Palomo, J. M.; Fernández-Lorente, G.; Guisán, J.M.; Fernández-Lafuente,R.
Partial and Enantioselective Asymmetric Hydrolysis of diethyl phenylmalonate by immobilized preparations of lipase from Thermomyces lanuginosa.
Enzyme Microb technol. 2007,40,1280-1285.

Cabrera, Z.; Lopez-Gallego, F.; Fernández-Lorente, G.; Palomo, J. M.; Montes, T.; Grazu, V.; Guisán, J.M.; Fernández-Lafuente,R.
Asymmetric hydrolysis of dimethyl  phenylmalonate by immobilized preparations of Penicillin G. acylase from e. coli.
Enzyme Microb Technol , 2007, 40,997-1000.

Fernández-Lorente, G.;Palomo, J. M.; Cabrera, Z.; Fernández-Lafuente,R.; Guisán, J.M.
Improved catalytic properties of  immobilized lipases by the presence of very low concentrations  of detergents in the reaction medium.
Biotechnol. Bioeng. 2007,  97,242-250.

 Mateo C.; Grazu, V.; Palomo J. M.;Lopez-Gallego, F.; Fernandez-Lafuente, R.; Guisán, J.M.
Immobilization of Enzymes on Heterofunctional Epoxy Supports.
Nature protocols. 2007, 2,1022-1033.

 Palomo, J. M.; Fernández-Lorente, G.; Fernández-Lafuente, R.;Guisán, J.M.
Modulation of the immobilized lipase enantioselectivity via chemical amination.
Adv. Synth Catal.2007, 349,1119-1127.

Fernández-Lorente, G.; Palomo, J. M.; Guisán, J.M. ; Fernández-Lafuente, R.
Effect of the immobilization protocol in the activity, stability and enantioslectivity of LECITASE® ULTRA.
J. Mol.Cat B: Enzym. 2007, 47,99-104.

Mateo C.; Palomo J. M.; Fernández-Lorente, G.; Fernandez-Lafuente, R.; Guisán, J.M.
Improvement of enzyme activity, stability and selectivity via immobilization techniques.
Enzyme Microb Technol. 2007,40 1451-1463.

Palomo, J. M*; Filice, M; Fernandez-Lafuente,R.; Terreni,  M.; Guisan, Jose M.
Regioselective hydrolysis of peracetylated β-monosaccharides by immobilized lipases. Key Role of the immobilization protocol.
Adv. Synth Cat. 2007,349,1969-1976

Fernández-Lorente,G., Palomo, J. M, Cabrera,Z.; Guisan, Jose M Fernandez-Lafuente,R.
Specificity enhancement towards hydrophobic substrates by immobilization of lipases by interfacial activation on hydrophobic supports.
Enzyme  Microb. Technol, 2007,41,565-569.

Filice, M; Fernandez-Lafuente,R.; Terreni,  M.; Guisan, Jose M, Palomo, J. M*.
Screening of Lipases for Regioselective Hydrolysis of peracetylated β-Monosaccharides.
J. Mol.Cat B: Enzym. 2007,49,12-17.

.Mateo C.; Grazu, V.; Pessela B.C.C., Montes, T., Palomo, J.M., Torres, R., Lopez-Gallego, F.; Fernandez-Lafuente, R.; Guisán, J.M.
Advances in the design of new epoxy supports for enzyme immobilization-stabilization.
Biochem.  Soc. Trans.2007,35, 1593–1601.

de Araújo A.D.; Palomo, J.M.; Cramer J.; Köhn,M.; Schröder,H , Wacker,R.; Niemeyer , C.; Alexandrov,K.; Waldmann H.
Diels-Alder Ligation and Immobilization of Peptides and Proteins.
Angew. Chem. 2006, 118,302-307.

.de Araújo A.D.; Palomo, J. M.; Cramer J.; Köhn,M.; Schröder,H , Wacker,R.; Niemeyer , C.; Alexandrov,K.; Waldmann H.
Diels-Alder Ligation and Immobilization of Peptides and Proteins.
Angew. Chem. Int. Ed. 2006,45,296-301. Highlights in Nature Methods

Palomo, J. M.; Lumbierres, M.; Waldmann ,H.
Efficient Solid-phase synthesis of lipopeptides employing the Ellman sulfonamide linker.
Angew. Chem. 2006, 118, 491-495.

Palomo, J. M.; Lumbierres, M.; Waldmann ,H.
Efficient Solid-phase synthesis of lipopeptides employing the Ellman sulfonamide linker.
Angew. Chem. Int. Ed. 2006, 45, 477-481.

Nicolini, C.; Baranski, J.; Schlummer, S.; Palomo, J.; Lumbierres-Burgues, M.; Kahms, M.; Kuhlmann, J.; Sanchez,J.; Gratton, E.; Waldmann, H.; Winter, R.
Visualizing association of N-Ras in lipid microdomains with two-photon fluorescence excitation and atomic force microscopy the influence of domain structure and interfacial adsorption .
J.Am.Chem.Soc.2006, 128, 192-201.

Palomo, J.M.; Segura, R.L.; Fuentes, M.; Ortiz, C.; Guisán, J.M.; Fernández-Lafuente,R
Unusual enzymatic resolution of racemic glycidyl-butyrate for the production of S-glycidyl derivatives.
Enzyme Microb Technol. 2006, 38, 429-435.

Lumbierres, M.;Palomo, J. M.; Kragol,G.; Waldmann ,H.
Solid-phase synthesis of palmitoylated and farnesylated lipopeptides employing the fluoride-labile PTMSEL linker.
Tetrahedron Lett. 2006, 47,2671-2674.

.Wilson, L.; Palomo, J.M.; Fernández-Lorente, G.; Illanes, A.; Guisán J.M.;. Fernández-Lafuente, R.
Improvement of functional properties of a thermostable lipase from alcaligenes sp. via strong adsorption on hydrophobic supports.
Enzyme Microb. Technol.2006,38,975-980.

Torres, R.; Ortiz, C.; Pessela, B.C.; Palomo, J.M.; Mateo, C.; Guisán, J. M.; Fernández-Lafuente,R.
Improvement of the enantioselectivity of lipase (fraction B) from Candida antarctica via adsorpiton on polyethylenimine-agarose under different experimental conditions.
Enzyme Microb. Technol. 2006, 39, 167-171.

Mateo, C.; Palomo, J.M.; Fuentes, M.; Betancor,L.; Grazu, V.; Lopez-Gallego, F.; Pessela, B.C.; Hidalgo, A.; Fernández-Lorente,G.; Fernández-Lafuente R.;. Guisán, J. M.
Glyoxyl-agarose:a fully inert hydrophilic support for immobilization and high stabilization of proteins.
Enzyme Microb. Technol. 2006, 39,274-280.

Wilson, L.; Palomo, J. M.; Fernández-Lorente, G.; Illanes, A.; Guisán, J. M;. Fernández-Lafuente, R.
Effect of lipase-lipase interactions in the activity,stability and specificity of a lipase from Alcaligenes sp.
Enzyme Microb. Technol.2006, 39,259-264

Wilson, L.; Fernández-Lorente, G.; Fernández-Lafuente, R.; Illanes, A.; Guisán, J.M.; *Palomo, J. M.
CLEAs of lipases and poly-ionic polymers: a simple way of preparing stable biocatalysts with improved properties.
Enzyme Microb. Technol. 2006, 39,750-755.

Segura, R. L.; Betancor, L.; Palomo, J. M.; Hidalgo,A.; Fernández-Lorente, G.;Terreni, M.; Mateo, C.; Cortés, A.; Fernández-Lafuente,R.;Guisán,J.M.
Purification and identification of the enzyme responsible of most esterasic activity in commercial porcine pancreatic lipase.
Enzyme Microb. Technol. 2006, 39, 817-823

.de Araújo A.D.; Palomo, J. M.; Cramer, J.; Seizt, O.; Alexandrov, K.; Waldmann H.
Diels-Alder Ligation and Immobilization of Peptides and Proteins.
Chem. Eur. J. 2006. 12, 6095-6109.

Fuentes, M.; Pessela, B.C.C.; Mateo, C.; Palomo, Jose M.; Batalla,P.; Fernández-Lafuente, R.; Guisán, Jose M.
Adsorption behaviour of bovine serum albumin on lowly activated anionic exchangers suggests a New strategy for solid–phase proteomics.
Biomacromolecules. 2006, 7,1357-1361.

Fernández-Lorente, G.;Palomo, Jose M.; Mateo,C.; Munilla, R.; Cabrera, Z.; Guisán, J.M.; Fernández-Lafuente, R.
Glutaraldehyde crosslinking in the presence of detergents of lipases adsorbed on aminated supports; Improving lipases performance.
Biomacromolecules. 2006, 7,2610-2615.

Palomo, J.M.; Fernández-Lorente, G.; Ortiz, C.; Segura, R.L.; Mateo, C.; Fuentes, M.; Hermoso, J.; Fernández-Lafuente, R; Guisán, J.M.
Conformational engineering of lipases via directed immobilization improving the resolution of chiral drugs.
Med. Chem. Rev.2005, 2,369-378.

Lumbierres, M., Palomo, J. M.; Kragol, G.; Roehrs, S.; Müller, O.; Waldmann, H.
Solid-phase synthesis of lipopeptides
Chem. Eur. J. 2005,11,7405-7415.

Fuentes, M.; Palomo, J.M.; Mateo,C.; Venteo, A.; Saenz, A.; Fernández-Lafuente R.; Guisán, Jose M.
Optimization of the modification of carrier proteins with aminated haptens
J. Immunol. Meth. 2005, 307, 144-149.

Palomo, J. M.;Segura, R.L.; Mateo,C.; Guisán,J.M.; Fernández-Lafuente,R.
Improving the activity of lipases from Thermus sp. at moderate temperatures for biotechnology applications.
Biomacromolecules. 2004, 5,249-254.                       

Fernández-Lorente, G.; Palomo, J. M.; Mateo, C.; Guisán, J. M.; Fernández-Lafuente,R.
Resolution of paroxetine precursor using different lipases.Influence of the reaction conditions on the enantioselectivity of lipases.
Enzyme Microb. Technol. 2004,34,264-269.

Palomo, J.M.; Segura, R.L.; Fernández-Lorente, G.; Guisán, J. M.; Fernández-Lafuente,R.
Enzymatic Resolution of (±)-glycidyl butyrate in aqueous media. Strong modulation of the properties of the lipase from Rhizopus oryzae via immobilization techniques.
Tetrahedron: Asymmetry.2004,15,1157-1161.

Mateo, C.; Palomo, J. M.; Van Langen, L.M.; Van Rantwijk, F.; Sheldon, R.
A new mild cross-linking methodology to prepare cross-linked enzyme aggregates.
Biotechnol. Bieng.2004, 86,273-276.

Palomo, J. M.; Segura, R. L.; Fernández-Lorente, G.; Pernas, M.; Rua, M.L.;Guisán, J.M.; Fernández-Lafuente,R.
Purification, Immobilization and Stabilization of a lipase from Bacillus thermocatenulatus by Interfacial Adsorption on Hydrophobic Supports.
Biotechnol Prog. 2004, 20,630-635.

Abian, O.; Mateo, C.; Palomo, J. M.; Fernández-Lorente, G.; Guisán, J.M.; Fernández-Lafuente,R.
Modulation of penicillin G Acylase properties by the acyl donor in enantioselectivity síntesis of phenylacetamides in the presence of high organic co-solvent concentration.
Biotechnol Prog.2004,30,984-988.

Segura, R.L.; Palomo, J. M.; Mateo,C.; Terreni, M.; Guisán, J. M.; Fernández-Lafuente,R.
Evaluation of three different fractions of lipases contained in pancreatic pig live extracts as enantioselective biocatalysts.
Biotechnol Prog. 2004,20,825-829.

Palomo, J. M; Ortiz, C.; Fuentes, M.; Fernández-Lorente,G.; Guisán, J.M; Fernández-Lafuente,R.
Use of immobilized lipases for lipase purification via specific lipase–lipase interactions.
J.Cromatogr.A.2004, 1038, 267-273.

Fuentes, M.; Pessela, B.C.C.; Maquiese, J.V; Ortiz, C.; Segura, R.L.;Palomo, J. M.; Abian, O; Guisán,J.M.; Mateo,C.; Fernández- Lafuente,R.
Reversible and Strong Immobilization of Proteins by Ionic Exchange on Supports Coated with Sulfate-Dextran
Biotech Prog.2004, 20, 1134-1139.

Kragol, G.; Lumbierres, M.; Palomo, J. M.; Waldmann,H.
Solid-Phase Synthesis of Lipidated Peptides.
Angew. Chem. Int Ed. 2004,43, 5839-5842.

Palomo, J.M ; Guisán, J. M.; Fernández Lafuente, R.
Solid-phase lipase engineering:a new tool in combinatorial biocatalysis.
Pharma Chem,2004, 3, 42-45.

Palomo, J.M.; Peña, M.; Fernández-Lorente, G.; Mateo, C.; Pisabarro, A.G.; Fernández-Lafuente, R.;Ramirez. L.; Guisán, J.M.
Solid Phase Handling of Hydrophobins: Immobilized Hydrophobins as a New Tool to Study Lipases.
Biomacromolecules.2003,4,204-210.

Palomo, J.M.; Muñoz, G.; Fernández-Lorente, G.; Mateo, C.; Fuentes, M.; Guisán J.M.; Fernández-Lafuente, R.
Modulation of Mucor miehei lipase properties via directed immobilization on different hetero-functional epoxy resins.Hydrolytic resolution of (R,S)-2-butyroyl-2-phenylacetic acid.
J. Mol. Cat B: Enzymatic.2003, 21,201-210.

Palomo, J. M.; Fuentes, M.; Fernández-Lorente, G.; Mateo,C.; Guisán, J.M.; Fernández-Lafuente,R.
General trend of Lipase to auto-assemble giving bi-molecular aggregates greatly modifies the enzyme functionality.
Biomacromolecules.2003,4,1-6.

Palomo, J. M.; Mateo,C.; Fernández-Lorente,G.; Solares , L.F.; Diaz, M.;. Sánchez, V.M.; Bayod,M.;
Gotor, V.; Fernández-Lafuente ,R.;Guisán , J.M.
Chiral Resolution of (R,S)-5-substituted-6-(5-chloropyridin-2-yl)-7-oxo-5,6-dihydropyrrolo[3,4b] pyrazine derivatives-precursors of (S)-(+)-Zopiclone catalyzed by immobilized Candida antarctica B lipase in aqueous media.
Tetrahedron: Asymmetry. 2003, 14, 429-438.

Fernández-Lorente, G.; Palomo, J. M.; Fuentes, M.; Mateo,C.; Guisán, J.M; Fernández-Lafuente, R.
Self-assembly of Pseudomonas fluorescens lipase into bimolecular aggregates dramatically affects functional properties.
Biotechnol.Bieng. 2003,82,232-237.

Mateo, C.; Torres,R.; Fernández-Lorente,G.; Ortiz, C.; Fuentes, M.; Hidalgo, A.; López-Gallego,F.;
Abian,O.; Palomo, J. M.; Betancor, L.; Pessela, B.C.C.; Guisán, J.M.; Fernández-Lafuente,R.
Epoxy-amino groups: a new tool for improved immobilization of proteins by the epoxy method.
Biomacromolecules. 2003,4,772-777.

Torres,R.; Mateo,C.; Ortiz,C.; Fuentes, M.; Palomo, J. M.; Guisán,J.M.; Fernández-Lafuente,R.
A novel heterofunctional epoxy-amino sepabeads for a new enzyme immobilization protocol: immobilization-stabilization of beta-galactosidase from A. oryzae.
Biotechnol.Prog. 2003, 19,1056-1060.

Fernández-Lorente, G.; Palomo, J. M.; Cocca, J.; Mateo, C.; Fernández-Lafuente, R.;
Moro, P.;Terreni ,M.; Guisán, J. M.
Regio-selective deprotection of peracetylated sugars via lipase hydrolysis.
Tetrahedron. 2003, 59,5705-5711.

Palomo, J. M.; Fernández-Lorente, G.; Rúa, M.L.; Guisán,J.M.; Fernández-Lafuente,R.
Evaluation of the lipase from Bacillus thermocatenulatus as an enantioselective biocatalyst.
Tetrahedron: Asymmetry.2003, 14,3679-3687.

Palomo, Jose M.; Fernández-Lorente, G.; Mateo,C.; Ortiz,C.; Fernández-Lafuente,R.; Guisán J,M.
Modulation of the enantioselectivity of lipases via controlled immobilization and medium engineering Hydrolytic resolution of Mandelic acid esters.
Enzyme Microb. Technol. 2002,31,775-783.

Palomo, Jose M.; Fernández-Lorente, G.; Mateo, C.; Fuentes, M.; Fernández-Lafuente, R.; Guisán, JM.
Modulation of the enantioselectivity of Candida antarctica B lipase via conformational engineering: kinetic resolution of (±)-alfa-hydroxy-phenylacetic acid derivatives.
Tetrahedron: Asymmetry. 2002,13,1337-1345.

Palomo, Jose M.; Fernández-Lorente, G.; Mateo,C.; Fuentes,M.; Guisán ,J.M.; Fernández-Lafuente, R..
Enzymatic production of (3S,4R)-4-(4´-fluorophenyl)-6-oxo-piperidin-3- carboxylic acid using a commercial preparation of lipase A from Candida antarctica: the role of a contaminant esterase.
Tetrahedron:Asymmetry.2002, 13,2653-2659.

Palomo, Jose M.; Fernández-Lorente,G.; Mateo,C.; Fernández-Lafuente ,R.; Guisán, J. M.
Enzymatic resolution of (±)-trans-4-(4´-fluorophenyl)-6-oxopiperidin-3-ethyl carboxilate, intermediate in synthesis of (-)-Paroxetine.
Tetrahedron: Asymmetry. 2002,13,2375-2381.

Palomo, Jose M.; Muñoz,G.; Fernández-Lorente, G.; Mateo, C.; Fernández-Lafuente,R.; Guisán, J.M.
Interfacial adsorption of lipases on very hydrophobic support (Octadecyl-Sepabeads): Immobilization, hyperactivation and stabilization of the open form of lipases.
J. Mol. Cat B: Enzymatic. 2002,19-20,279-286.

Abian, O.; Wilson, L.; Mateo, C.; Fernández-Lorente, G.; Palomo, J.M.; Fuentes,M.; Fernández-Lafuente, R.;
Guisán, J.M.;Re, D.; Tam, A.; Daminatti, M.
Preparation of artificial hyper-hydrophilic micro-environments (polymeric salts) surrounding immobilized enzyme molecules. New enzyme derivatives to be used in any reaction medium.
J. Mol. Cat B: Enzymatic. 2002,19-20,295-303.

Torres,R.; Mateo,C.; Fuentes, M.; Palomo, J. M.; Ortiz,C.; Fernández-Lafuente ,R.;
Guisán, J.M.; Tam,A.; Daminati, M.
Reversible Immobilization of Invertase on Sepabeads Coated with Polyethyleneimine: Optimization of the Biocatalyst’s Stability.
Biotechnol. Prog.2002, 18,1221-1226.

Fernández-Lafuente, R.; Mateo,C.; Abian, O.; Fernández-Lorente, G.; Palomo, J. M.; Fuentes,M.; Guisán, J. M.
Industrial synthesis of semi-synthetic beta-lactam antibiotics: recent developments in enzyme biocatalysis for improved and more sustainable proceses.
Curr. Med. Chem.: Anti-infective agents. 2002,1,375-387.